KDM2A plays a dual role in regulating the expression of malignancy-related genes in esophageal squamous cell carcinoma

KDM2A is a histone demethylase, which primarily catalyzes the demethylation of H3K36me2. Abnormal expression of KDM2A is observed in many types of cancers; however, the molecular events connected to KDM2A expression remain unclear. In this work, to gain further insight into the molecular mechanism underlying the function of KDM2A, we analyzed the expression and function of KDM2A in esophageal squamous cell carcinoma (ESCC) cells. In this study, 20 paired samples from patients that underwent ESCC resection were used to verify the expression of KDM2A by IHC. Subsequently, the KDM2A-specific shRNA was transfected into Kyse150 and TE11 cell lines using a lentivirus system. The growth curving, CCK8, colony formation and wound healing assays were utilized to access the proliferation and migration capacities. RNA-seq was performed to evaluate the downstream targets of KDM2A.