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SpyCLIP: An easy-to-use and high-throughput compatible platform for efficient characterization of protein-RNA interactions

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NIAID Data Ecosystem2026-03-11 收录
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We have developed a covalent linkage-based SpyCLIP method by taking advantage of SpyTag-SpyCatcher chemistry, which circumvents gel purification steps of captured RBP-RNA needed for all existing CLIP methods, thereby dramatically reducing the technical difficulty and enabling high-throughput application of the CLIP technology. SpyCLIP and corresponding input sample for PTBP1, SLBP, RBFOX2 and AGO2 in Lenti-X 293T cell line.

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2019-04-17
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