CROP-Seq in Primary Human T Cells. CROP-Seq in Primary Human T Cells

We adapted sgRNA lentiviral infection and Cas9 electroporation (SLICE) to allow for CROP-Seq (Datlinger et al, Nat Med 2017) in primary cells. We used a library of 48 sgRNA, derived from GW screens, to explore transcriptional changes downstream of CRISPR-KO. Overall design: Pooled CRISPR screen with single cell RNASeq readout (Perturb-Seq / CROP-Seq) in primary human T cells. Dataset includes CD8 T cells from two donors, for two conditions: with TCR stimulation or No stimulation.