Trajectory Mapping of the Early Drosophila Germline Reveals Controls of Zygotic Activation and Sex Differentiation

We report single-cell RNA-sequencing data for sexed D. melanogaster germ cells from 0-8h embryos. We find in this time window that the transcriptome of male and female germ cells diverge and this coincides with the activation of the zygotic germline genome. Three datasets were collected to provide comprehensive coverage of the early germline: 0-8h unsexed germ cells, 5-8h male germ cells, 5-8h female germ cells