Subcommissural organ (SCO) contributes to brain development via SCO-secreted peptides

To explore the role of SCO and its functional mechanism, we performed RNAseq between SCO and hippocampus at different stage to identify the expression of some unique genes and possible pathway related to the function of SCO. We confirmed the role of SCO in neuronal development and identified three SCO-derived peptides which can contributed to neurite development and neuronal survival . Differential expression analysis and Gene Set Enrichment Analysis of RNA seq data. We performed differential expression analysis of RNA seq data obtained from SCO and hippocampus of adult mice and Gene Set Enrichment Analysis obtained from SCO and hippocampus of P0 mice.为探究SCO（Subcommissural Organ）的功能及其作用机制，我们针对不同发育阶段的SCO与海马组织开展了RNA测序（RNAseq）分析，以筛选与SCO功能相关的特异性基因表达谱及潜在调控通路。本研究证实了SCO在神经元发育中的核心作用，并鉴定出3种源自SCO的活性肽，其可促进神经突生长与神经元存活。我们对RNA测序数据开展了差异表达分析与基因集富集分析（Gene Set Enrichment Analysis, GSEA），其中针对成年小鼠的SCO与海马组织RNA测序数据进行差异表达分析，针对出生后0天（P0）小鼠的SCO与海马组织RNA测序数据进行基因集富集分析。