Whole genome sequencing (WGS) data from invasive pine sawfly Diprion similis

Biological introductions are unintended “natural experiments” that provide unique insights into evolutionary processes. Invasive phytophagous insects are of particular interest to evolutionary biologists studying adaptation, as introductions often require rapid adaptation to novel host plants. However, adaptive potential of invasive populations may be limited by reduced genetic diversity—a problem known as the “genetic paradox of invasions”. One potential solution to this paradox is if there are multiple invasive waves that bolster genetic variation in invasive populations. Evaluating this hypothesis requires characterizing genetic variation and population structure in the invaded range. To this end, we assemble a reference genome and describe patterns of genetic variation in the introduced white pine sawfly, Diprion similis. This species was introduced to North America in 1914, where it has rapidly colonized the thin-needled eastern white pine (Pinus strobus), making it an ideal invasi..., Full methods on how this data was prepared and processed can be found at the linked publication. In brief: Raw genetic material was collected from larval tissue from insects and extracted using Qiagen kits. DNA libraries were prepared using KAPA HyperPrep kits and was then sequenced on NovaSeq 6000 S4 flowcell. vcftools and ANGSD programs were used to prepare the sam and beagle files respectively. See the paper methods for more details. ,生物引种（Biological introductions）是无意形成的"自然实验"，可为进化过程研究提供独特见解。入侵植食性昆虫（invasive phytophagous insects）是研究适应性的进化生物学家尤为关注的类群，因为引种过程往往要求它们快速适应全新的寄主植物。然而，入侵种群的适应性潜力可能会因遗传多样性降低而受限——这一问题被称为"入侵遗传学悖论（genetic paradox of invasions）"。破解这一悖论的潜在方案之一，是存在多轮入侵事件以提升入侵种群的遗传变异水平。验证这一假说，需要对入侵区域内的遗传变异与种群结构进行表征分析。为此，我们组装了一套参考基因组，并对引入的白松叶蜂（Diprion similis）的遗传变异模式进行了描述。该物种于1914年被引入北美，随后快速定殖于针叶纤细的东部白松（Pinus strobus），使其成为理想的入侵…… 关于该数据集的制备与处理的完整方法，可参见关联发表的论文。简要而言：实验所用的原始遗传材料取自昆虫幼虫组织，使用Qiagen试剂盒（Qiagen kits）提取DNA。使用KAPA HyperPrep试剂盒构建DNA文库，随后在NovaSeq 6000 S4流动槽（NovaSeq 6000 S4 flowcell）上完成测序。分别使用vcftools与ANGSD程序处理生成sam文件与beagle文件。更多细节可参见论文中的方法部分。