DNA extraction and virome processing methods strongly influence recovered human gut viral community characteristics

This project examines how common methodological approaches impact the recovery of viral communities from the human gut. We generated shotgun sequencing libraries from a single human fecal sample using four workflows: total metagenomes, viromes without DNase treatment, DNase-treated viromes, and DNase-treated viromes subjected to multiple displacement amplification (MDA). These data enable comparisons of viral community profiles generated by each method.