Helicobacter pylori Rif1 Genome sequencing. Helicobacter pylori Rif1

Mutant strain Rif1 (from reference strain 26695) H. pylori reference strain 26695 was routinely maintained on Columbia agar (bioMerieux, France) plates supplemented with 10% heat-inactivated Horse Serum (PAA Lab., Austria), 5% yeast extract, and H. pylori-selective antibiotic mix Dent (Oxoid). Liquid culture was grown in BHI broth (3.7% brain heart infusion) (bioMerieux, France) with 10% inactivated Fetal Bovine Serum (HyClone, USA) and 5% yeast extract. Both plates and broth cultures were incubated at 37 under an atmosphere enriched with 5% CO2 for 2-3 days. The mutant strain Rif1 was selected by culturing H. pylori 26695 on selective plates containing 20 μgml of rifampicin (Sigma). In 5 days, resistant colony was picked and passed under rifampicin pressure.