Analysing the effect of cellular energy levels on codon-specific ribosome occupancy in vivo

The aim of this study is to evaluate how cellular energy levels shape codon-specific decoding and affect codon-mediated mRNA degradation. To this end we made use of HT5P-seq (Zhang & Pelechano, 2021, PMID:35474692) in S. cerevisiae to footprint the ribosome of co-translationally degraded mRNAs in vivo upon swift changes in the concentration of intracellular ATP and other energy metabolites.本研究旨在探究细胞能量水平如何调控密码子特异性解码过程，并影响密码子介导的信使核糖核酸（mRNA，messenger RNA）降解。为此，我们在酿酒酵母（Saccharomyces cerevisiae）中采用HT5P-seq技术（Zhang & Pelechano, 2021, PMID:35474692），对细胞内三磷酸腺苷（ATP，adenosine triphosphate）及其他能量代谢物浓度快速变化时，体内共翻译降解的mRNA的核糖体进行足迹分析。