Reprogramming of rhythmic liver metabolism by intestine clock

To assess potential impact of intestine clock on the rhythmicity of peripheral tissues (liver, kidney and WAT), mice with Bmal1 specifically deleted in the intestine (Bmal1-iKO mice, showing loss of clock function in the intestine) were generated. We collected liver, kidney and WAT samples from Bmal1-iKO and control (Bmal1-flox) mice every 4 hours throughout a light/dark cycle, and subsequently performed transcriptomic analyses. Comparative gene expression profiling analysis of RNA-seq data for Bmal1-iKO and Bmal1-flox mice.为评估肠道生物钟（intestinal clock）对外周组织（肝脏、肾脏及白色脂肪组织（white adipose tissue, WAT））节律性的潜在影响，我们构建了肠道特异性敲除Bmal1的小鼠（Bmal1-iKO小鼠，其肠道生物钟功能丧失）。我们在光暗周期内每4小时采集Bmal1-iKO小鼠与对照（Bmal1-flox）小鼠的肝脏、肾脏及WAT样本，随后开展转录组分析，并针对二者的RNA-seq数据进行比较性基因表达谱分析。