CUT&Tag analysis of human iPSCs differentiating into contracting striated muscle cells for the histone marks (H3K4me1, H3K4me3, H3K27ac, H3K27me3) and the transcription factor binding sites (HES1, HEYL, MYOD1, MYOG, PAX3, PAX7)

We differentiated human iPSCs in vitro into contracting striated muscle cells using the method as reviewed by Yan et al. 2021 (https://doi.org/10.1016/j.semcdb.2021.04.017), see also Chal et al. 2015, 10.1038/nbt.3297; Chal et al. 2016, 10.1038/nprot.2016.110; Al Tanoury et al. 2020, 10.1242/dev.187344; Al Tanoury et al. 2021, 10.1073/pnas.2022960118. At the time points day08, day12, and day20 of the primary differentiation as well as at the endpoint of the secondary differentiation, we used the CUT&Tag method (Kaya-Okur et al. 2019; 10.1038/s41467-019-09982-5) to map the the histone marks (H3K4me1, H3K4me3, H3K27ac, H3K27me3) and the transcription factor binding sites (HES1, HEYL, MYOD1, MYOG, PAX3, PAX7) in two biological replicates (*_R1 and *_R2). We deposit here the normalized BigWig files of the respective binding sites that have been generated from the respective *.bam files after genome-wide alignment to the hg38 build.