Kinetics of selected blood biomarkers of articular cartilage in response to stress test with modulated ambulatory load

The goal of this work was to determine the suitability of selected blood biomarkers of articular cartilage as mechanosensitive markers and to investigate the dose-response relationship between ambulatory load magnitude and marker kinetics in response to load.

Serum samples were collected from 24 healthy volunteers before (0) and at three time points (1=0min; 2=30min; 3=60min) after a 30-minute walking stress test performed on a treadmill on three test days. In each experimental session, one of three ambulatory loads was applied: 100% body weight (BW); 80%BW; 120%BW. Serum concentrations of COMP, MMP-3, MMP-9, ADAMTS-4, PRG-4, CPII, C2C and IL-6 were assessed using commercial enzyme-linked immunosorbent assays.

ADAMTS - A disintegrin and metalloproteinase with thrombospondin motifs

C2C - COL2−3/4Clong mono epitope

COMP - cartilage oligomeric matrix protein

CPII - C-propeptide is removed from the procollagen extracellularly and directly reflects the rate of type II procollagen synthesis

IL - interleukin

MMP - matrix metalloproteinasis

PRG - proteoglycan

The file contains the raw concentrations for each marker, participant, time point and condition.