Comparing different sample handling conditions for 16S rRNA amplicon sequencing

We examined a combination of sample handling choices (sample storage, extraction kit and 16S amplification) for swab material using an even mock community (HM-280). In addition, blank controls were used to monitor the level of laboratory and kit-specific contamination. By examining a combination of effects, we aim to contribute to the ongoing discussion regarding standardization of sample handling for amplicon and metagenomic sequencing.