Effects of daridorexant on gene expression in the hypothalamus of LPS-induced mice.

Acute inflammatory processes disrupt hypothalamic homeostatic systems, leading to a preference for sleep over wakefulness. Orexin knockout mice exhibit faster recovery from LPS-induced sickness, possibly due to enhanced sleep during inflammation. The reduction in orexin activity limits physical activity and encourages sleep, though it remains uncertain whether these responses are pathological or protective. In this study, to investigate the effects of daridorexant, a dual orexin receptor antagonist, on brain inflammation and sleep quality, we employed a systemic inflammatory model in C57BL/6J mice. LPS treatment resulted in significant decreases in REM sleep and wakefulness, while non-REM sleep increased. Here, we provide gene expression profiling of hypothalamus tissues using the RNA sequencing for identifying changes by daridorexant in gene expression of the LPS-treated mice. Overall design: mRNA profiles of hypothalamus were generated by RNA sequencing using the Illumina NextSeq 500 (Illumina).