Data from: A high-content imaging approach to profile C. elegans embryonic development

The C. elegans embryo is an important model for analyzing mechanisms of
cell fate specification and tissue morphogenesis. Sophisticated lineaging
approaches for analyzing embryogenesis have been developed but are
labor-intensive and do not naturally integrate morphogenetic readouts. To
enable the rapid classification of developmental phenotypes, we developed
a high-content method that employs two custom strains: a Germ Layer strain
expressing nuclear markers in the ectoderm, mesoderm and endoderm/pharynx,
and a Morphogenesis strain expressing markers labeling epidermal cell
junctions and the neuronal cell surface. We describe a procedure that
allows simultaneous live imaging of development in 80-100 embryos and
provide a custom program that generates cropped, oriented image stacks of
individual embryos to facilitate analysis. We demonstrate the utility of
our method by perturbing 40 previously characterized developmental genes
in variants of the two strains containing RNAi-sensitizing mutations. The
resulting datasets yielded distinct, reproducible signature phenotypes for
a broad spectrum of genes involved in cell fate specification and
morphogenesis. Our analysis additionally provides new in vivo evidence for
MBK-2 function in mesoderm fate specification and LET-381 function in
elongation.