mRNA-seq of zebrafish treated with sex hormone 17β-estradiol and aromatase inhibitor exemestane during sex development

To identify key genes in the early sex development of zebrafish, we generated zebrafish (AB strain) feminized by sex hormone 17β-estradiol (E2, CAS: 50-28-2, 100 ng/L) and masculinized by aromatase inhibitor exemestane (EM, CAS: 107868-30-4, 10 μg/L), and detected their transcriptomes by RNA-seq. PolyA+ RNA-seq were performed on 56 samples of four exposure treatments and five exposure periods, with 2-3 replicates at each exposure treatment and period. Each sample contained 25 zebrafish. The exposure treatments included (1) DMSO (100 μg/L), (2) EM + DMSO, (3) E2 +DMSO, and (4) EM+ E2 + DMSO.