Optimisation of HTT-HAP40 purification using heparin affinity chromatography - 2019/04/02
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https://zenodo.org/record/2628063
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资源简介：
Project: Biophysical investigation of purified HTT protein samples
Experiment: Optimisation of HTT-HAP40 purification using heparin affinity chromatography
Date completed: 2019/04/02
Rationale: Previous attempts to generate a much purer and homogenous HTT-HAP40 sample showed that the complex can bind heparin resin. In addition, I will incorporate helpful suggestions from scientists at the CHDI Palm Springs meeting full-length HTT research breakout group i.e. ATP wash to remove HSP proteins. The experiment will include 3 affinity chromatography steps with FLAG, heparin and NiNTA resin and finally a gel filtration step.
创建时间：
2024-07-24
