Dicamptodon copei microsatellite genotype data

Dicamptodon copei tissue samples were collected from the field in 2006-2008. DNA was extracted using DNeasy tissue kits (Qiagen) according to the manufacturer’s protocols. Eleven microsatellite loci were amplified using PCR with fluorescently labeled primers, using PCR conditions from Steele et al. (2008) (Table S1, Supporting Information). Microsatellite products were run on an ABI 3730XL automated sequencer (Applied Biosystems) at the Washington State University LBB1 core facility and genotyped using ABI GENEMAPPER 3.7 software. The data include microsatellite fragment lengths for each study site (rows) and (diploid) locus (columns).科氏钝口螈（Dicamptodon copei）的组织样本于2006至2008年间野外采集。采用Qiagen公司的DNeasy组织试剂盒（DNeasy tissue kits），按照厂商提供的操作流程提取基因组DNA。使用荧光标记引物，参照Steele等（2008）的PCR反应条件（详见补充材料表S1），对11个微卫星位点（microsatellite loci）进行扩增。扩增得到的微卫星产物于华盛顿州立大学LBB1核心实验室，使用应用生物系统公司（Applied Biosystems）生产的ABI 3730XL全自动测序仪进行电泳检测，并通过ABI GENEMAPPER 3.7软件完成基因分型。本数据集包含各研究样地（行）与（二倍体）位点（列）对应的微卫星片段长度数据。