CUT&Tag for H2A.Z using mouse heart tissues from control and Znhit1-deficient mice

Znhit1 is an important subunit of the SRCAP complex, which regulates gene transcription by replacing histone H2A with a variant H2A.Z at gene promoter regions. Deletion of Znhit1 has also been reported to impact the incorporation of H2A.Z in genome. To explore the function of Znhit1 in heart tissues, we performed CUT&Tag assays for H2A.Z to examine the distribution changes of H2A.Z on the genome. Overall design: CUT&Tag assays for H2A.Z using mouse heart tissues from control (Znhit1 flox/flox) and Znhit1 cKO (Znhit1 flox/flox; Myh6-Cre) mice at postnatal day 17 (P17) and postnatal day 25 (P25).