Transcriptome analysis of subacute injured kidney by intraperitoneal injections of Fe-NTA in wildtype and BRCA1+/- rats

Fenton reaction-based repeated oxidative stress via ferric nitrilotriacetate (Fe-NTA) causes renal cell carcinoma (RCC), which was found to be promoted in BRCA1 haploinsufficiency rats.To elucidate the molecular mechanism, we performed a subacute study of repeated ip administration of Fe-NTA for 1 or 3 weeks to understand the early events. We compared the microarray gene expression profiles of rat kidney cortex tissues of wild-type and BRCA1+/- rats from the study. The subacute kidney injury protocol was started with 17 wildtype and 18 BRCA1+/- male Jcl: SD rats. A total of 16 microarrays (Whole Rat Genome Microarray) were used for screening purpose: 8 microarrays for wildtype SD rats, and 8 for BRCA1+/- SD rats.