Gene expression changes in neonatal and adult plasmacytoid dendritic cells (pDC) upon TLR7/8 stimulation

We want to define the differences in gene expression changes between neonatal and adult plasmacytoid dendritic cells upon stimulation with TLR7/8. These differences may point to specific signaling pathways which, in turn, may account for the known differential immune response of babies to vaccination or infection when compared to adults. Total RNA obtained from MACS-purified human pDC (using Miltenyi's Diamond Plasmacytoid Cell Isolation Kit) stimulated with the synthetic TLR7/8 ligand 3M-003 for 1 or 6 hours in vitro compared to unstimulated pDC.