Summary of basic characteristics of the ten transgenic lines.

The GFP and RFP fluorescence intensity in the brain was examined under the fluorescent microscope and different intensities of the fluorescence were graded from the weakest “+” to the strongest “+++++”. The brain weights are expressed as ratios of the average from double transgenic mice to that of the age-matched wild type mice. The average weights with standard deviations and the statistics are shown in Table S1. The levels of mRNA and miRNA were determined by real-time RT-PCR. The mRNA levels of the double transgenic mice are presented as a percentage of the wild type mice. The miRNA levels of the double transgenic mice are presented as fold increases over the background value obtained from wild typemice. The transgene copy numbers were determined by real-time PCR of the genomic DNA from single transgenic miRNA lines. The different types of junctional fragments were detected by PCR using specific primer pairs (see Table 3). Stg: single transgenic mice positive for the G/R-miRNA transgene only.Dtg: double transgenic mice positive for both the G/R-miRNA transgene and nestin-Cre. Wt: non-transgenic mice. Br: brain. Wgt: weight ratio of Dtg-to-Wt. Stg%Wt: level in Stg as a percent of Wt. Dtg%Wt: level in Dtg as a percent of Wt. Tg: transgenic. H-T: head-to-tail.H-H: head-to-head. T-T:tail-to-tail. “+” indicates positive for the specific junctional PCR product. “−” indicates negative for the specific junctional PCR product. “+*” indicates that this PCR product sequence was successfully read (see Fig. 2).