The effects of atorvastatin (AVS) and afidopyropen on developing zebrafish

For RNA-seq experiments, 20 embryos were placed into 5 mL final volume of sterile ERM. Each treatment group were exposed within a 10-minute window. Exposure groups included ERM, [100 ng/L AVS or 1 µg/L AVS] or [100 ng/L or 1 µg/L afidopyropen] (N=5 beakers per treatment). On 7 days post fertilization (dpf), fish were transferred to a labeled and pre-weighed 1.7 mL tube. The tube was then immediately flash frozen in liquid nitrogen prior to RNA-seq analysis. Exposure groups included 5 treatments: ERM, [100 ng/L AVS or 1 µg/L AVS] or [100 ng/L or 1 µg/L afidopyropen] Four replicates out of the 5 were ultimately selected for RNA-seq.