Root microbes can improve plant tolerance to insect damage: A systematic review and meta-analysis

To limit damage from insect herbivores, plants rely on a blend of defensive mechanisms that includes partnerships with beneficial microbes, particularly those inhabiting roots. While ample evidence exists for microbially mediated resistance responses that directly target insects through changing phytotoxin and volatile profiles, we know surprisingly little about the microbial underpinnings of plant tolerance. Tolerance defenses counteract insect damage via shifts in plant physiology that reallocate resources to fuel compensatory growth, improve photosynthetic efficiency, and reduce oxidative stress. Despite being a powerful mitigator of insect damage, tolerance remains an understudied realm of plant defenses. Here we propose a novel conceptual framework that can be broadly applied across study systems to characterize microbial impacts on expression of tolerance defenses. We conducted a systematic review of studies quantifying the impact of rhizosphere microbial inoculants on plant toler..., A meta-analysis was performed following systematic review of the literature. We identified papers from our primary literature that fit the following criteria: (1) examined interactions between at least one insect herbivore, one root microbe, and one plant, (2) considered herbivore damage that was real rather than simulated (i.e., mechanical damage), or mechanical damage supplemented with insect regurgitant, (3) applied microbial inoculants to plant roots or soil (i.e., excluded cases of strictly shoot inoculation), (4) focused on the root microbial community (i.e., not phyllosphere/aerial plant tissues), (5) considered at least one measure of plant tolerance (e.g., yield, plant biomass, oxidative stress mitigation, or photosynthesis), (6) included no-microbe control treatments with which to compare treatments that added one or more microbes, and (7) considered wild-type varieties/cultivars, rather than mutant genotypes. The resultant 40 papers that fit these criteria are summarized in t..., , # Root microbes can improve plant tolerance to insect damage: A systematic review and meta-analysis [https://doi.org/10.5061/dryad.v15dv425n](https://doi.org/10.5061/dryad.v15dv425n) ## Description of the data and file structure # Microbial-Mediated-Tolerance-MetaAnalysis This repository contains the data files and analysis code for completion of the meta-analysis and summary of systematic review. # Data Files - .csv download three data files to run the analysis code \"all-papers-summary.csv\"Â \"hedge-d-simple-ContinMod.csv\"Â \"tolerance lit review table_working1august.csv\" # Analysis code - .Rmd \"meta-analysis-forGitHub_Final.Rmd\" ### Files and variables #### File: all-papers-summary.csv **Description:**Â Summary of variables and information collected from studies included in literature review and meta-analysis. This data file was used for analysis and works with R code provided. Empty cells indicate a measurement was not recorded or data is missing. ##### Variables * Citat...为抵御植食性昆虫（insect herbivores）为害，植物依赖一系列防御机制，其中包括与有益微生物（beneficial microbes）建立共生关系，尤其是定殖于根系的微生物。尽管已有大量证据表明，微生物介导的抗性响应可通过改变植物毒素（phytotoxin）与挥发性物质谱（volatile profiles）直接靶向昆虫，但我们对植物耐性的微生物基础（microbial underpinnings）却知之甚少。耐性防御通过调控植物生理过程重新分配资源以支持补偿生长（compensatory growth）、提升光合效率（photosynthetic efficiency）并减轻氧化应激（oxidative stress），从而抵消昆虫为害造成的损失。尽管耐性是缓解昆虫为害的有效手段，但该领域仍是植物防御研究中尚未得到充分探索的方向。在此，我们提出一套新颖的概念框架，可广泛应用于各类研究系统，以表征微生物对植物耐性防御表达的影响。我们针对量化根际微生物菌剂（rhizosphere microbial inoculants）对植物耐性影响的研究开展了系统综述（systematic review）……本研究在完成系统综述后开展了元分析（meta-analysis）。我们从原始文献中筛选出符合以下标准的论文：(1) 至少包含一种植食性昆虫、一种根系微生物与一种植物的互作研究；(2) 采用真实的植食性昆虫为害（而非模拟为害，即机械损伤），或辅以昆虫反刍液（insect regurgitant）的机械损伤处理；(3) 将微生物菌剂施加于植物根系或土壤（排除仅进行地上部接种（shoot inoculation）的案例）；(4) 聚焦于根系微生物群落（而非叶围（phyllosphere）/植物地上组织）；(5) 至少测定一项植物耐性相关指标（如产量、植物生物量、氧化应激缓解或光合作用）；(6) 设置无微生物的对照处理，用于与添加一种或多种微生物的处理组进行比较；(7) 采用野生型品种/品系（wild-type varieties/cultivars）而非突变体基因型（mutant genotypes）进行实验。最终符合上述标准的40篇论文的相关内容已在……中进行了总结…… , # 根系微生物可提升植物对植食性昆虫为害的耐性：一项系统综述与元分析 [https://doi.org/10.5061/dryad.v15dv425n](https://doi.org/10.5061/dryad.v15dv425n) ## 数据与文件结构说明 # 微生物介导耐性元分析 本存储库包含完成本次元分析与系统综述总结所需的数据文件及分析代码。 # 数据文件——.csv格式 需下载以下三个数据文件以运行分析代码："all-papers-summary.csv" "hedge-d-simple-ContinMod.csv" "tolerance lit review table_working1august.csv" # 分析代码——.Rmd格式 "meta-analysis-forGitHub_Final.Rmd" ### 文件与变量 #### 文件：all-papers-summary.csv **描述**：本文件汇总了纳入本次文献综述与元分析的研究中的变量与采集信息。该数据文件可配合提供的R代码开展分析，空单元格代表未记录对应测量值或数据缺失。 ##### 变量 * Citat...