qPCR data analysis.pzf
收藏Figshare2022-02-26 更新2026-04-08 收录
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https://figshare.com/articles/dataset/qPCR_data_analysis_pzf/19242561/1
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资源简介：
<b>Raw qPCR data and data analysis file</b>Expression profile of (a) hsa-miR-143-3p, (b) its predicted targets, (c) stem cell markers, (d) differentiation marker and (e) Wnt signaling regulators upon transfection with hsa-miR-143-3p mimic and inhibitor. Relative mRNA / miRNA expression in mimic transfected and inhibitor transfected cells were quantified in comparison to control by qPCR using SYBR Green chemistry. Each sample (n = 3) was run in triplicate. The data were expressed as mean ± SD and relative fold change of expression (RQ) was calculated by 2<sup>-∆∆CT</sup> method after normalization with GAPDH (reference gene)/ RNU6B (reference miRNA). p < 0.05 was considered to be statistically significant.<br>
提供机构：
Daniels, Julie; Muthukkaruppan, Veerappan; Prajna, Venkatesh; Chidambaranathan, Gowri Priya; Kalaimani, Lavanya; Devarajan, Bharanidharan
创建时间：
2022-02-26
