qPCR data analysis.pzf

<b>Raw qPCR data and data analysis file</b>Expression profile of (a) hsa-miR-143-3p, (b) its predicted targets, (c) stem cell markers, (d) differentiation marker and (e) Wnt signaling regulators upon transfection with hsa-miR-143-3p mimic and inhibitor. Relative mRNA / miRNA expression in mimic transfected and inhibitor transfected cells were quantified in comparison to control by qPCR using SYBR Green chemistry. Each sample (n = 3) was run in triplicate. The data were expressed as mean ± SD and relative fold change of expression (RQ) was calculated by 2^-∆∆CT method after normalization with GAPDH (reference gene)/ RNU6B (reference miRNA). p &lt; 0.05 was considered to be statistically significant.<br>