Quantitative phytoplankton profiles for Pensacola Transect Station P1 collected during the R/V Bellows cruise of DeSoto Canyon, April 6, 2013
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This dataset reports phytoplankton species abundance data for samples collected on 2013-04-06 onboard the R/V Bellows BE_1317 cruise in the Gulf of Mexico from 2013-04-06 to 2013-04-08. Samples were taken at Pensacola Transect Station P1 (30.25o N, 87.25o W) in the Florida Panhandle Bight at the head of DeSoto Canyon. One-liter samples were collected from the surface and a depth of 18 meters using Niskin bottles. Up to one liter of each sample was filtered onto a 0.45-micrometer nitrocellulose filter and air-dried. A portion of each filter was mounted on an aluminum stub, sputter-coated, and examined with a JEOL JSM-6480 LV scanning electron microscope. Diatoms, coccolithophores, and armored dinoflagellates were identified to the lowest possible taxonomic category. The number of cells of each taxon in a field of view at a magnification of 1000x was recorded, with up to 700 fields-of-view examined, depending on the density of phytoplankton in the sample. The dataset includes the depth, the volume filtered, the number of cells of each taxon counted, and the calculated abundance of each taxon in cells per liter. Micrographs taken during the analysis are included.
本数据集记录了2013年4月6日采集的样品对应的浮游植物物种丰度数据,样品采集工作依托“Bellows号”科考船(R/V Bellows)执行的BE_1317航次开展,该航次在墨西哥湾的作业时段为2013年4月6日至4月8日。采样站位为位于德索托峡谷头部的佛罗里达潘汉德尔湾彭萨科拉断面P1站(30.25°N,87.25°W)。研究人员使用尼斯金采水器(Niskin bottles)采集表层及18米水深的1升水样。每份样品取至多1升体积,经0.45微米硝酸纤维素滤膜(nitrocellulose filter)过滤后风干。将部分滤膜安装于铝制样品台,经喷镀处理后,使用JEOL JSM-6480 LV型扫描电子显微镜(scanning electron microscope)进行观测。对硅藻、颗石藻(coccolithophores)及具壳甲藻(armored dinoflagellates)开展了最低可行分类阶元水平的鉴定。在1000倍放大倍数下记录每个视野内各分类群的细胞数,依据样品中浮游植物的密度差异,最多可观测700个视野。本数据集包含采样水深、过滤体积、各分类群的计数细胞数,以及以每升细胞数计算得到的各分类群丰度值,同时收录了分析过程中拍摄的显微照片。



