scRNA-seq of human cerebral organoids derived from healthy and FOXG1 syndrome patients iPSCs at d105

Disruptions in gene expression programs during nervous system development?affecting both transcriptional and post-transcriptional regulation?are implicated in a range of neurodevelopmental disorders. Among these, FOXG1 syndrome remains poorly understood, particularly with respect to the mechanistic role of its namesake gene, FOXG1. In this study, we performed single-cell RNA sequencing (scRNA-seq) on day 105 (d105) cerebral organoids derived from healthy controls as well as from iPSCs carrying FOXG1^del and FOXG1^c.460dupG mutations, to investigate cell type?specific gene expression changes. Overall design: Single-cell RNA sequencing (scRNA-seq) was performed on day 105 (d105) human cerebral organoids derived from healthy controls (2 replicates) and FOXG1-syndrome patient-derived iPSCs representing two genotypes (2 replicates each). The patient-specific mutations include the FOXG1^del and FOXG1^c.460dupG frameshift mutations.