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Guide RNAs containing universal bases enable Cas9/Cas12a recognition of polymorphic sequences

NIAID Data Ecosystem2026-03-13 收录

CRISPRi基因编辑

Cas12a靶向识别

数据链接：

https://www.ncbi.nlm.nih.gov/sra/SRP289686 数据链接链接失效反馈

官方服务：

资源简介：

CRISPR/Cas9 guide RNAs Raw sequence reads

应用场景：

创建时间：

2021-11-26

病毒宿主因子筛选

CRISPRi基因编辑

Human choriocarcinoma JAR4 clone, an MAVS-gene- and PKR-gene-knockout derivative from JAR cells (ATCC HTB-144), was infected with lentiviral pools of human GeCKOv2 CRISPR knockout pooled library (Addg

NIAID Data Ecosystem100

Additional file 2 of Repression of HIV-1 reactivation mediated by CRISPR/dCas9-KRAB in lymphoid and myeloid cell models

CRISPRi基因编辑

细胞功能研究

Additional file 2: 98 design sgRNA from CRISPRPick tool.

Figshare2022-06-23 更新30

Table2_Targeted Knockout of Eukaryotic Translation Initiation Factor 4E Confers Bymovirus Resistance in Winter Barley.XLSX

大麦抗病毒育种

CRISPRi基因编辑

The Eukaryotic Translation Initiation Factor 4E (EIF4E) is a well-known susceptibility factor for potyvirus infections in many plant species. The barley yellow mosaic virus disease, caused by the bymo

NIAID Data Ecosystem20

Screening_Zebrafish_for_CRISPR_induced_indels. Screening_Zebrafish_for_CRISPR_induced_indels

CRISPRi基因编辑

斑马鱼突变筛选

Zebrafish embryos were injected with CRISPR reagents, collected at 48hours and loci corresponding to the CRISPRs were amplified by PCR and libraries were made by the addition of tailed oligos in a sec

NIAID Data Ecosystem50

File S2.zip

CRISPRi基因编辑

基因敲入序列分析

Annotated sequence files in .ape format (comparable with Genbank) of knock-ins generated by CRISPR/Cas9-mediated genome editing. Unmodified genomic sequence is also provided.

Figshare2023-03-14 更新20

Guide RNAs containing universal bases enable Cas9/Cas12a recognition of polymorphic sequences

资源简介：

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