File S1 - WAVE3-NFκB Interplay Is Essential for the Survival and Invasion of Cancer Cells

File contains Figures S1-S9. Figure S1. NFκB promoter luciferase activity in TNFα treated versus untreated cells: Luciferase-based NFκB reporter assay in MDA-MB-231 (A) and BT549 (B) cells before and after treatment with TNFα. MDA-MB 231 cells were transfected with NFκB reporter, negative and positive controls, along with a Renilla luciferase reporter construct for internal normalization. After 24 h, cells were treated with 50 ng/ml of recombinant human TNFα for 15 min. Dual luciferase assays were performed in a 96-well plate and promoter activity values are expressed in arbitrary units after normalization to luciferase activity of the Renilla reporter. Experiments were performed in triplicates, and the standard deviation is indicated. All data are representative of 3 independent experiments, or are the mean (±SE; n = 3; *, p (PDF)