Reprogramming of flagellin receptor responses with surrogate ligands

Characterization of the genome-wide transcriptional responses induced by flg22, Maya1 and Maya2 using RNA sequencing. Total RNA from leaves and roots of wildtype (WT) and fls2 mutant plants were obtained as follows. For leaves, 4-weeks-old Arabidopsis plants grown on soil were treated for 2 hours with DMSO, flg22, Maya1, or Maya2. Total RNA was isolated from pools of more than 15 leaves isolated from independent plants. For roots, 5-day-old seedlings grown vertically on plates were treated for 1 hour with flg22 or Maya2. After 1 hour of incubation, the root tips including the meristematic zone, elongation zone, and a small portion of the maturation zone were isolated. More than 30 independent roots were pooled to extract total RNA for each sample. All the experiments were repeated three times (n=3) except for flg22 treatment in roots (n=2). Details about libraries construction and sequencing strategy are appended below.