Intestinal mRNA profiles of 2-month old control (APCmin) and APCmin; Setd2IEC-/- mice generated by deep sequencing

Setd2 catalyzes trimethylation of lysine 36 on histone H3. H3K36me3 is deposited mainly in the gene body and has recently been demonstrated to play a role in regulating transcriptional elongation and alternative splicing. We conduct deep sequencing in 2-month old control (APCmin) and APCmin; Setd2IEC-/- mice intestinal cells to understand the splicing events regulated by Setd2. Intestinal mRNA profiles of 2-month old control (APCmin) and APCmin; Setd2IEC-/- mice were generated by deep sequencing. Each RNA sample was from 3 littermates RNA mixture.Setd2可催化组蛋白H3第36位赖氨酸的三甲基化修饰。H3K36me3主要沉积于基因体区域，近期研究证实其可调控转录延伸与可变剪接过程。为阐明Setd2所调控的剪接事件，本研究对2月龄对照（APCmin）小鼠与APCmin; Setd2IEC-/- 小鼠的肠道细胞进行了深度测序。本研究通过深度测序获得了2月龄对照（APCmin）及APCmin; Setd2IEC-/- 小鼠的肠道mRNA表达谱。每份RNA样本均来自3只同窝仔鼠的RNA混合样品。