Cep135 deficiency promotes cell cycle de-regulation, p53 accumulation and cell deah of neural progenitors

Gene expression profiles of Cep135 wildtype and knockout E14.4 and E11.5 mouse embryonic neocortices. Gene expression profiles of in vitro E14.5 cortices-derived primary neurospheres are also included; in vitro samples listed as "12h" or "24h" depending on the duration of the in vitro culture from NPs isolation for incipient neurosphere formation and RNA isolation for RNAseq. E11.5 and E14.5 embryos resulting from matings between cep135 (+/-) mice were sacrificed and dorsal telencephalons dissected out to RNA isolation and RNAseq analysis. In the case of E14.5 telencephalons, E14.5 cortical neural progenitors were also isolated and cultured for 12 and 24h for the formation of incipient neurospheres. RNAseq analysis of these in vitro cultures is also available.本数据集收录了Cep135野生型与基因敲除（knockout）型的E14.4、E11.5小鼠胚胎新皮层的基因表达谱。此外还纳入了体外培养的E14.5皮层来源原代神经球（primary neurospheres）的基因表达谱；体外样本依据从分离神经前体细胞（neural progenitors, NPs）以启动初始神经球形成，到为RNA测序（RNAseq）提取RNA的体外培养总时长，标注为"12h"或"24h"。本研究通过Cep135杂合子（cep135 (+/-)）小鼠交配获得E11.5与E14.5胚胎，处死后剥离其背侧端脑（dorsal telencephalons）用于RNA提取与RNAseq分析。针对E14.5端脑，研究者同时分离其皮层神经前体细胞并体外培养12h、24h以诱导初始神经球形成，亦对上述体外培养样本完成了RNAseq分析。