An aging-like phenotype occurs in Tif1gamma-/- hematopoietic stem cells

To determine whether an accelerated aging-like phenotype occurs in hematopoiesis of young Tif1gamma-/- mice (4 months old), we purified 200,000 hematopoietic stem cells (LSK: Lineage negative, Sca1+, c-Kit+) from Tif1gamma-/- mice and performed high-throughput mRNA sequencing (RNA-seq). We compared this transcriptome to physiological aging by creating two other RNAseq libraries from young (4 months old) and old (20 months old) wild type mice. RNAseq study on young Tif1gamma-/- mice (4 months old), young wild type mice (4 months old) and old wild type mice (20 months old).为探究4月龄年轻Tif1γ纯合缺失（Tif1gamma-/-）小鼠的造血系统是否存在类加速衰老表型，我们从该类小鼠体内纯化得到200000个造血干细胞（LSK：谱系阴性、Sca1阳性、c-Kit阳性），并开展高通量mRNA测序（RNA-seq）。为将该转录组与生理性衰老的转录组进行比对分析，我们额外分别从4月龄年轻野生型（wild type）小鼠与20月龄老年野生型小鼠中构建了两组RNA测序文库。本研究的测序对象涵盖4月龄年轻Tif1gamma-/-小鼠、4月龄年轻野生型小鼠及20月龄老年野生型小鼠。