The miRNA profile of P. xylostella in response to chlorantraniliprole exposure

miRNAs play important roles in various biological processes through post-transcriptional regulation of gene expression. We previously identified 203 mature miRNAs in Diamondback moth, Plutella xylostella. This species has developed extremely high levels of resistance to chlorantraniliprole and other class of insecticides in the field. In this study, we examined the miRNA profile of P. xylostella in response to chlorantraniliprole exposure. The smRNA-seq data analyses showed that insecticide treatment caused significant changes in the abundance of some miRNAs. Increasing exposure time (6h to 24h) and insecticide concentration (0.01 to 0.1 ppm) induced more dysregulated miRNAs in DBM larvae. To determine P. xylostella miRNA responses to a xenobiotic stress, we exposed the susceptible DBM larvae to two doses of Chlorantraniliprole (0.01 and 0.1 ppm). Total RNA samples were collected from surviving larvae 6 and 24 h post-treatment. This included 16 samples (three replicates for each time and dose of treatment plus two replicates of control larvae at both times).