Enterococcus faecium Genome sequencing and assembly

During the last 2 decades, VREhave become significant nosocomial pathogens worldwide,mainly due to their adaptability in hospital environments and thelimited treatment options. Nine types of glycopeptide resistancedeterminants (vanA, vanB, vanC, vanD, vanE, vanG, vanL, vanM,and vanN) have been reported and well characterized on the basisof phenotypic and genotypic criteria.We first reported the vanM gene in a vancomycin-resistantEnterococcus faecium (VREm) clinical isolate from a teaching hospitalin Shanghai in 2006. Subsequently, only a single studyfrom Singapore has reported vanM-type VRE isolates.Therefore we performed whole genome sequencing of three Enterococcus faecium isolates to validate the way of vanM transduction using bioinformatics.