NAP1L2 Drives Mesenchymal Stem Cell Senescence and Suppresses Osteogenic Differentiation [RNA-Seq]

Changes in these genes are probably a consequence of aging, and the real regulators governing BMSC senescence and osteogenesis are still unclear.In the current study, we report that nucleosome assembly-related protein NAP1L2 serves as an important regulator of both the senescence and osteogenic differentiation of BMSCs through inhibition of NF-κB signaling and recruitment of SIRT1 to deacetylate the H3K14ac level on promoters of osteogenic genes. Thus, targeting NAP1L2 using an aging antagonist such as NMN would benefit aging-related disease. Non-target control and knockdown Nap1l2 C3H10T1/2 cells were divided into the following four groups depending on whether osteogenesis induction for 7days is performed: NC, KD-Nap1l2, NC-7day, KD-Nap1l2-7day.And cells were harvested for gene expression profiling. Two replicates were performed.