Additional file 2 of Regulation of β-cell death by ADP-ribosylhydrolase ARH3 via lipid signaling in insulitis

Additional file 2: Table S1. Characteristics of human islet donors. Table S2. Data collection parameters. Table S3. List of antibodies and oligonucleotides used in the study. Table S4. Quantitative proteomics analysis of islets from NOD and NOR mice at 6 weeks of age. Table S5. Differentially abundant proteins found in the quantitative proteomics analysis of islets from NOD and NOR mice at 6 weeks of age. Table S6. Quantitative lipidomics analysis in the negative-ionization mode of the EndoC-βH1 cells (n = 4) treated with 50 U/mL IL-1β + 1000 U/mL IFN-γ for 48 h. Table S7. Quantitative lipidomics analysis in the positive-ionization mode of the EndoC-βH1 cells (n = 4) treated with 50 U/mL IL-1β + 1000 U/mL IFN-γ for 48 h. Table S8. Quantitative lipidomics analysis in the negative-ionization mode of the human islets (n = 10) treated with 50 U/mL IL-1β + 1000 U/mL IFN-γ for 24 h. Table S9. Quantitative lipidomics analysis in the positive-ionization mode of the human islets (n = 10) treated with 50 U/mL IL-1β + 1000 U/mL IFN-γ for 24 h. Table S10. Quantitative lipidomics analysis in the negative-ionization mode of islets from 6-weeks old NOD vs. NOR mice. Table S11. Quantitative lipidomics analysis in the positive-ionization mode of islets from 6-weeks old NOD vs. NOR mice. Table S12. Quantitative proteomics analysis of wild-type and Pla2g6 knockdown MIN6 beta cells treated with cytokines (100 ng/mL IFN-γ, 10 ng/mL TNF-α, and 5 ng/mL IL-1β) for 24 h. Table S13. Identification of proteins that the abundances are regulated by cytokines via PLA2G6. Wild-type and Pla2g6 knockdown MIN6 beta cells treated with cytokine cocktail CT2 (100 ng/mL IFN-γ, 10 ng/mL TNF-α, and 5 ng/mL IL-1β) for 24 h were analyzed by proteomics. The criteria for selecting PLA2G6-dependent cytokine-regulated proteins were: (i) significantly regulated by the cytokine treatment (untreated wild type vs. wild type treated with cytokines), (ii) this regulation also had to be significant comparing wild type treated with cytokines vs. Pla2g6 knockdown treated with cytokines, and (iii) not significantly changing in untreated Pla2g6 knockdown vs. Pla2g6 knockdown treated with cytokines.