E. coli Sacculi +A22 (MG1655)

Tilt Series Date: 2015-04-10 Data Taken By: Matt Swulius Species / Specimen: Escherichia coli Strain: MG1655 sacculi Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x. Microscope: Caltech Polara Acquisition Software: UCSFTomo Upload Method: pipeline Processing Software Used: raptor Sample Preparation: Cells were treated for one hour with 10ug/ml A22 until they were rounded and lemon-shaped prior to sacculus purification.倾斜序列采集日期：2015-04-10 数据采集者：Matt Swulius 物种/样本：大肠杆菌（Escherichia coli） 菌株：MG1655 肽聚糖囊（sacculi） 倾斜序列采集参数：单轴倾斜，倾斜范围(-60.0°，60.0°)，步长1°，恒定角度增量，电子剂量150.0 eV/Ų，欠焦量-6.0 μm，放大倍数22500× 显微镜型号：Caltech Polara 采集软件：UCSFTomo 上传方式：流水线上传 所用处理软件：raptor 样本制备流程：将细胞以10 μg/mL的A22溶液处理1小时，待细胞变为圆形及柠檬状后，再进行肽聚糖囊纯化。