RQ fused cassette raw data for Pseudomonas aeruginosa isolates PS1 and PS26

The first column describes the P. aeruginosa isolates (PS1 and PS26) and the gene targeted in the quantitative PCR for measuring their transcription (gcu14; GES-1; aacA4; and the fusion GES-1-aacA4 and rpsL), which was performed in triplicate. Also in this column are the negative controls for each PCR reaction (NTC). The second column displays the cycle threshold (CT), i.e., the PCR cycle in which the fluorescence was detected by the machine. The third and the fourth columns refer to the standard deviation and the average of CT values, respectively, between triplicates. The fifth column refers to the to the normalization of the target gene transcript amount relative to that of endogenous gene (rpsL). The sixth column corresponds to the results of relative quantification. For example, the gcu14 gene and GES gene in PS1 had RQ values of 15,88 and 7,80. It means that gcu14 is 2-fold more transcribed than GES in PS1 isolate.

本列描述了铜绿假单胞菌分离株（PS1和PS26）及其在定量PCR中针对转录进行的靶向基因（gcu14；GES-1；aacA4；以及GES-1-aacA4融合基因和rpsL）的检测，该检测以三份重复进行。此外，本列还包含了每项PCR反应的阴性对照（NTC）。第二列展示了循环阈值（CT），即机器检测到荧光的PCR循环数。第三列和第四列分别表示三份重复之间CT值的标准差和平均值。第五列涉及目标基因转录量相对于内源基因（rpsL）的标准化。第六列对应于相对定量结果。例如，在PS1分离株中，gcu14基因和GES基因的相对定量值分别为15.88和7.80。这意味着在PS1分离株中，gcu14基因的转录量是GES基因的两倍。