Efficient High-Throughput Discovery of Large Peptidic Hormones and Biomarkers

A novel approach is presented for the simultaneous identification and relative quantification of secreted peptides, particularly those that have been historically difficult to analyze in a concerted manner. Peptides exceeding 60 residues with various degrees of post-translational modification were identified on a liquid chromatographic time scale. The approach demonstrates high efficiency pattern-based recognition analysis of complex neuroendocrine peptide sets and enables rapid identification of biomarkers from biological material. Keywords: CART • chromogranin • hybrid FT−MS • insulinoma • pattern recognition • peptidomics • top down proteomics