Figure S2 - Correction: In Vivo Expression Technology Identifies a Novel Virulence Factor Critical for Borrelia burgdorferi Persistence in Mice

The BBK46 protein is non-immunogenic in mice. Recombinant GST-BBK46 and GST alone produced in and purified from E. coli, along with total protein lysate from E. coli and B. burgdorferi (Bb lysate) and E. coli producing the B. burgdorferi antigen BmpA (+) were separated by SDS-PAGE and transferred to a nitrocellulose membrane. Immunoblot analysis was performed using immune serum collected from mice infected with wild-type B. burgdorferi and anti-GST monoclonal antibodies (α GST). The positions of markers to the left of the panel depict protein standard molecular masses in kilodaltons. (TIF)