ESKAPEE BLAST nucleotide database (rMAP-2.0)

This record provides a pre-built BLAST nucleotide database archive for the ESKAPEE bacterial pathogens used by rMAP-2.0. The archive contains a complete makeblastdb output directory (index and auxiliary files) so users can run blastn queries without rebuilding the database locally. Contents eskapee_db.tar.gz — compressed tar archive containing the BLAST database files (e.g., .nsq, .nin, .nhr, and related files) under eskapee_db/ eskapee_db.tar.gz.sha256 — SHA-256 checksum for integrity verification How the database was builtThe database was generated from a curated FASTA file of ESKAPEE bacterial sequences using NCBI BLAST+: makeblastdb \ -in eskapee_db.fasta \ -dbtype nucl \ -parse_seqids \ -max_file_sz 3000000000 \ -out eskapee_db/eskapee_db Download and unpack # download from Zenodo and verify checksum sha256sum -c eskapee_db.tar.gz.sha256 # unpack tar -xzvf eskapee_db.tar.gz Example usageAfter unpacking, the database prefix is: eskapee_db/eskapee_db Example blastn query: blastn -query query.fasta -db eskapee_db/eskapee_db -outfmt 6 -max_target_seqs 10 -evalue 1e-10 > blast_hits.tsv Intended useThis database archive is intended to support reproducible, rapid local BLAST-based screening within the rMAP-2.0 workflow and related microbial genomics analyses, especially in settings where rebuilding large databases is time-consuming or bandwidth-limited. VersioningThis Zenodo record corresponds to version of the ESKAPEE BLAST database used in rMAP-2.0. Updated databases will be released as new versions on Zenodo. Project repositoryThe rMAP-2.0 code and documentation are available on GitHub: [GitHub repo link / rMAP-2.0] (add as a “Related identifier” in Zenodo). ChecksumSHA-256 checksum is provided in eskapee_db.tar.gz.sha256 and should be used to validate file integrity after download.