Supplemental data: Variations in salivary oxytocin and eye caruncle temperature indicate valuing of environmental enrichment material in fattening pigs

This document provides supplemental data and respective R script to the manuscript "Variations in salivary oxytocin and eye caruncle temperature indicate valuing of environmental enrichment material in fattening pigs" submitted to the journal Applied Animal Behaviour Science on January 11, 2023. The study reports an experimental study exploring the potential of physiological and behavioral measures to non-invasively detect valuing responses to environmental enrichment in fattening pigs. Our results illustrate that lachrymal caruncle temperature, measured by infrared thermography, salivary oxytocin, and play behavior can vary in response to short-term changes to provision of fresh straw. This study represents a step toward the development of non-invasive measures to investigate anticipation and, consequently, affective states in pigs and the progress of the study of animal emotions. The experimental procedures were carried out in accordance with the Ministry of Food, Agriculture and Fisheries, The Danish Veterinary and Food Administration under act 474 of 15. May 2014 and executive order 2028 of 14. December 2020, and under consideration of the ARRIVE Guidelines (Du Sert et al., 2020). The study was developed as part of project PIGWEB funded by the European Union’s Horizon 2020 research and innovation program under grant agreement No. 101004770.本文件为2023年1月11日提交至《应用动物行为科学》（Applied Animal Behaviour Science）期刊的稿件《唾液催产素与眼泪阜温度变化可反映育肥猪对环境丰容（environmental enrichment）材料的重视程度》提供补充数据及对应R脚本。 本研究为一项实验研究，旨在探索通过生理与行为指标非侵入式检测育肥猪对环境丰容的重视反应的可行性。研究结果显示，经红外热成像技术（infrared thermography）测量的泪阜温度、唾液催产素水平以及玩耍行为，会因新鲜秸秆供给的短期变动而发生变化。本研究为开发非侵入式指标以探究猪的预期及后续情感状态，以及推动动物情感研究的进展迈出了重要一步。 本实验操作严格遵循丹麦食品、农业与渔业部及丹麦兽医与食品管理局2014年5月15日第474号法案与2020年12月14日第2028号行政命令，并符合ARRIVE指南（Du Sert等，2020）的相关要求。本研究作为PIGWEB项目的一部分开展，该项目由欧盟地平线2020（Horizon 2020）研究与创新计划资助，资助协议编号为101004770。