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Method for constructing rice molecular marker map based on Kompetitive Allele Specific PCR and application in breeding Using the same

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国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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The present invention provides a method for constructing a rice molecular marker map based on Kompetitive Allele Specific PCR (KASP) and application in breeding using the map, belonging to the field of agricultural technology. In the present invention, genome-wide recombination sequencing is conducted on rice and recombination sequencing data SNPs are mined for searching SNP sites; combined with existing SNP databases, 565 SNP sites required for breeding are obtained based on the SNP sites; and the rice molecular markers map is constructed by molecular marking with the selected SNP sites. The SNP markers of the constructed rice molecular marker map have the advantages of clear physical location and simple and convenient detection method, which can be directly used for molecular identification and genetic background analysis of some important agronomic traits, and has important application value for improving rice molecular breeding efficiency.
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国家林业和草原科学数据中心
创建时间:
2023-02-12
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