Transcriptome profile of S. cerevisiae strains expressing mistranslating tRNAs

Mistranslation, the mis-incorporation of an amino acid not specified by the “standard” genetic code, occurs in all cells. tRNA variants that increase mistranslation arise spontaneously and engineered tRNAs can achieve mistranslation frequencies approaching 10% in yeast and bacteria. The goal of this study was to assess the transcriptome changes in yeast cells expressing two different mistranslating tRNA variants which mistranslate at similar frequencies (of ~ 3%) but result in different substitutions, namely alanine at proline codons or serine at arginine codons. Overall design: RNA sequencing of yeast strains expressing tRNA-Pro(G3:U70) or tRNA-Ser(UCU, G26A) compared to a wild-type yeast strain expressing no mistranslating tRNA