Sampling of planktonic microorganisms for metagenomic, metaproteomic and metatranscriptomic analyses

Large volumes of water (200 - 500 L) were filtered and fractionated by size for various planktonic components: eukaryotic phytoplankton, prokaryotic picoplankton, and marine viruses. Sample sites were chosen to generate the widest diversity, and included planktonic blooms, oligotrophic zones, small polynyas near sea ice, nearshore areas, and Antarctic bottom water from coastal, canyon and deepwater areas. Half of each sample will be used for DNA library construction, and the other half will be used for meta-proteomic analysis. Random shotgun sequencing of the marine genomic libraries should produce a metagenomic snapshot of planktonic life in a variety of marine habitats. This work was completed as part of ASAC project 2899 (ASAC_2899).本数据集通过对200~500升的大量海水进行尺寸分级过滤，分离得到不同的浮游生物组分：真核浮游植物、原核超微型浮游生物（picoplankton）以及海洋病毒。 采样点的选择旨在覆盖最广泛的生物多样性，涵盖浮游生物水华区域、寡营养海域、海冰周边小型冰间湖、近岸区域，以及取自沿岸、峡谷与深水区域的南极底层水。 每份样品将均分两份，一半用于DNA文库构建，另一半用于宏蛋白质组学分析。 对该海洋基因组文库开展随机鸟枪法测序，可获取多种海洋生境中浮游生物群落的宏基因组快照。 本研究作为ASAC项目2899（ASAC_2899）的一部分完成。