We have found that some B. pertussis isolates exhibit autoagglutination (Agg) phenotype when cultured on a synthetic cyclodextrin solid medium. The Agg did not occur in the presence of MgSO4, Nicotini
This is from the transposon mutant library of Pseudomonas aeruginosa PAO1. The aim is to check for the presence of PL sub complexes in the absence of FliF protein.
AD1231: pAD1361/A136; Carbenicillin 50; virB, virD4, virGN54D on multicopy plasmid, host lacks a Ti-plasmid. Constitutive virG mutant, does not require AS. But good to have AS in the media, anyway.
