Local administration of IFN-a-iPSC-pMCs alters gene expression profiles in tumor microenvironment.

Local administration of IFN-a-producing proliferating myeloid cells (IFN-a-iPSC-pMCs) inhibited the tumor growth not only at the treatment site (right) but also at the distant site (left). We identified genes with log fold expression change = 1 or = -1 in untreated versus IFN-a-iPSC-pMC-treated tumors and iPSC-pMC-treated versus IFN-a-iPSC-pMC-treated tumors for treatment or distant site, respectively. Of the overlapping genes differentially expressed in IFN-a-iPSC-pMC-treated mice, 301/671 distant site and 460/928 treated site genes were ISGs, of which 95.3% and 94.7%, respectively, were type I IFN-related. By gene ontology, the up-regulated ISG signatures were enriched for “T cell-mediated immune responses”, “cytolysis”, and “migration of immune cells” associated genes. Collectively, Local administration of IFN-a-iPSC-pMCs alters the tumor microenvironment and propagates molecular signature associated with type I IFN. Overall design: Gene expression profiles of treated tumor (right) and distant tumor (left)