Morphometric Analyses of Uveal Melanomas with QuPath

Extraction of nuclear features by computational method from high resolution whole-slide digital scans of histologic slides has been shown to be a valuable tool. Our study utilized QuPath, a free, open-source software and program for whole-slide image analysis, which allows cell detection and cell type classification (such as tumor cells, stromal cells, necrotic or apoptotic cells, and immune cells). Additionally, QuPath enables analysis of large numbers of tumor cells and generates detailed cell and nuclear features (nuclear area, nuclear diameter, and cell circularity). In our study, morphometric analyses have been performed on 80 uveal melanomas from Tumor Genome Cancer Atlas (TGCA) which have available virtual slides, clinical and genomic information.